한국어업기술학회:학술대회논문집 (Proceedings of the Korean Society of Fisheries Technology Conference)
- 한국어업기술학회 2000년도 춘계수산관련학회 공동학술대회발표요지집
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- Pages.487-488
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- 2000
RAPD Analysis of DNA Polymorphism and Genetic Species-Specificity Using PCR Technique in the Marine Microalgae
- Yoon, Jong-Man (Department of Marine Biomedical Science, Kunsan National University) ;
- Chang, Kye-Nam (West Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
- 발행 : 2000.05.01
초록
Genomic DNA was isolated from the marine microalgae representing genetic characteristics and genomic polymorphisms by polymerase chain reaction amplification of DNA as arbitrary primers. The electrophoretc analysis of PCR-RAPD products showed hig levels of variation between different genus and little variation between different species. Outer of these primers, 6 generated 248 highly reproducible RAPD markers, producing almost seven polymorphic bands per primers. The degree of similarity frequency between Chaetoceros gracilis and Chaetoceros calcitrans species showed 90% as calculated by sharing analysis. The RAPD polymorphism generated by this primer may be used as a genetic marker for genus or species identification in important marine microalgae. (omitted)
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