인공 항문계를 이용한 대두박 isoflavones의 생체 이용성 평가

Soybean meal(SMB) has not only been an important source of protein, but also a major source of isolfavones(IF) for poultry diet. Since the IF is one of effective phytoestrogens, evaluation of bioavailability of the phytochemicals for poultry and development of an animal model for assessment of the availability should be essential for its practical application. Present study was to develop bird model for assessing their bioavailability. No reports for such measurments with poultry could have every been found. Eight, 6-wk-old, layer-type, male birds were divided into two groups of four birds; normal (N) and colostomized(COL). Each bird was tube-fed a SMB sample at a level of 1% body weight. Samples from blood, urine and feces(COL birds) or urine+feces (N) were collected at 0, 2, 8, 15, 24 and 48 hours after the feeding. Plasma and urine samples were treated with -glucuronidase and fecal samples with 4N HCl for measurement of genistein(GE) and daidzein (DA) with HPLC set with an electrochemical detector. Maximum peaks of plasma IF appeared at 2 h after the meal for both birds groups. However the IF peak for N group was GE, while that for COL group was DA. Plasma DA levels for COL birds were always higher than those of GE, which was in opposite trend for N birds. This trend observed in the N birds is in accordance with those reported in rats fed SBM. In conclusion the results seem to suggest that colostomy as an attempt to separate urine from feces in birds affected the IF metabolism in the intestine and may not be a proper bird model to measure the IF bioavailability.