한국가금학회:학술대회논문집 (Proceedings of the Korea Society of Poultry Science Conference)
- 한국가금학회 1999년도 제16차 정기총회및학술발표회
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- Pages.11-33
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- 1999
닭에서 원시생식세포의 효율적 분리 및 외래 유전자 전이에 관한연구
Studies on the Efficient Separation of Primordial Germ Cells and Introduction of Foreign DNA in the Chicken
초록
This study was conducted to determine the embryonic stages for the isolation of the highest number of PGCs and to improve PGCs enrichment method. The primordial germ cells(PGCs) from different sources of chick embryos were isolated. The embryonic stage having the highest number of PGCs from each sources was selected ; 1-day-old embryos for germinal crescent (stage 6-8), 2.5-day-old embryos for blood (stage 17-18) and 5.5-day-old embryos for gonad (stage 27-28). The number of PGCs from one embryonic germinal crescent, blood and gonad was about 87
키워드