Structure Elucidation of Analgesic Constituents from Yerba Buena Leaves

Three analgesic constituents: FB2c, FB6Fc, and FB10E5c from the hexane extract of Mentha cordifolia Opiz. (Yerba buena) leaves were isolated by solvent partitioning and sequential repeated vacuum liquid chromatography. Spectral analysis of the three constituents show that FB2c is ${\beta}$-sitosterol; FB10E5c is ${\beta}$-sitosteryl-${\beta}$-D-glucopyranoside; and FB6Fc is a cis-8- pentadecenyl with lactone variety. At a dosage of 100 mg/kg mouse, isolates FB2c, FB6Fc, and FB10E5c decreased the number of squirms induces by acetic acid by 70.0 %, 67.3 %, and 73.0 %, respectively.