Picosecond Protein Fluorescence and Time-Resolved $Eu^{3+}$ Luminescence Spectroscopic Studies on the Roles of $Ca^{2+}$ in Subtilisin Carlsberg

  • Lee, Sunbae (Department of Chemistry, Seoul National University) ;
  • Jang, Du-Jeon (Department of Chemistry, Seoul National University)
  • 발행 : 1997.07.01

초록

Ca$^{2+}$ is one of the most common metal ions associated with proteins, playing more or less well-defined functional roles in biological activities. In protease, the presence of $Ca^{2+}$ slows down autolysis and enhances thermal stability. Subtilisin, one of the best studied proteases, is known to have two $Ca^{2+}$ -binding sites.(omitted)

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