Proceedings of the KOSOMBE Conference (대한의용생체공학회:학술대회논문집)
- Volume 1996 Issue 05
- /
- Pages.335-338
- /
- 1996
Reconstruction of basement membrane in the artificial skin
인공피부 제조시 기저막 재건의 효과
- Yi, J.Y. (Lab of Cell Biology, Korea Cancer Center Hospital, KAERI) ;
-
Park, K.C.
(Department of Dermatology, Seoul National University College of Medicine)
;
-
Kim, S.H.
(Department of Plastic Surgery, Seoul National University College of Medicine)
;
- Suh, H. (Department of Biomedical Engineering, Yonsei University) ;
- Son, Y.S. (Lab of Cell Biology, Korea Cancer Center Hospital, KAERI)
- 이재연 (한국 원자력 연구소 부설 원자력 병원, 세포 생물학 연구실) ;
-
박경찬
(서울대학교 의과대학 피부과)
;
-
김석화
(서울대학교 의과대학 성형외과)
;
- 서활 (연세대학교 의과대학 의공학 교실) ;
- 손영숙 (한국 원자력 연구소 부설 원자력 병원, 세포 생물학 연구실)
- Published : 1996.05.17
Abstract
We attempted to reconstruct basement membrane (BM) in between the epidermal compartment and dermal compartment in the artificial skin preparation and examine its effect on the skin architecture as well as on the epidermal differentiation. Laminin, one of the component of BM, stimulate the migration of the basal cells but type IV collagen which is a major component of the mechanical network of BM did not stimulate epidermal migration. However laminin in the presence of type IV collagen at a 1:1 molar ratio did not stimulate epidermal migration but provide nice demarcation between epidermis and dermis. This mixture of laminin and type IV collagen enhanced epidermal differentiation in the artificial skin based on the morphological observation as well as biochemical criteria. The epidermal acquirement of migratory ability on the laminin-rich substrate suggest that this type of unbalance in the expression of the components of BM may prevail in the area of healing tissue and the invasive transition of the tumor. The result in this study provide the technical improvement in the artificial skin preparation and further application of this technique for the reconstruction of other bio-artificial organ.
Keywords