대한의용생체공학회:학술대회논문집 (Proceedings of the KOSOMBE Conference)

대한의용생체공학회 (The Korean Society of Medical and Biological Engineering)
권호 표지

Redistribution of Intracellular Calcium Stores with Shear Stress-induced Cytoskeleton Organization in Human Endothelial Cell

  • 정찬일 (서울대학교 의대 의공학교실 및 의공학연구소) ;
  • 장현아 (서울대학교 의대 의공학교실 및 의공학연구소) ;
  • 장준근 (서울대학교 공대 정밀기계설계연구소 및 기계설계학과) ;
  • 한동철 (서울대학교 공대 정밀기계설계연구소 및 기계설계학과) ;
  • 민병구 (서울대학교 의대 의공학교실 및 의공학연구소)
  • Chung, Chan-Il (Dept. of Biomedical Engineering, Seoul National University) ;
  • Chang, Hyun-A (Inst. of Biomedical Engineering, Seoul National University) ;
  • Chang, Jun-Keun (Inst. of Biomedical Engineering, Seoul National University) ;
  • Han, Dong-Chul (Inst. of Advanced Machinery and Design, Seoul National University) ;
  • Min, Byoung-Goo (Dept. of Biomedical Engineering, Seoul National University)
  • 발행 : 1996.05.17
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초록

Intracellular gradients of the free calcium concentration are thought to be critical for the localization of functional responses within a cell. The mechanism of mechanotransduction may be associated with the localized accumulation of calcium stores for shear stress-exposed endothelial cells. The distribution of the calcium stores and the formation of the stress fibers were investigated by the indirect double immunofluorescent staining method with the calreticulin antibody and rhodamine phalloidin under flow condition. The shear stress of steady flow reorganized the cytoskeleton structure including the bundling and translocation to focal contacts. The calcium stores translocated from the cytoplasm to the focal contacting area. Consequently. accumulation of the calcium stores may participate in the shear stress-induced cytoskeleton organization of endothelial cells.

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