한국생물물리학회:학술대회논문집 (Proceedings of the Korean Biophysical Society Conference)
- 한국생물물리학회 1996년도 정기총회 및 학술발표회
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- Pages.33-33
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- 1996
Structural Analysis of the Ectodomain of HIV Gp41 and Implication on the Gp41 Assisted Membrane Fusion
- Ryu, Jae-Ryen (Structural Biology Center, Korea Institute of Science and Technology) ;
- Lee, Jung (Structural Biology Center, Korea Institute of Science and Technology) ;
- Suh, Mu-Jin (Doping Control Center, Korea Institute of Science and Technology) ;
- Yu, Yeong-Sook (Doping Control Center, Korea Institute of Science and Technology) ;
- Yu, Yeon-Gyu (Structural Biology Center, Korea Institute of Science and Technology)
- 발행 : 1996.07.01
초록
An ectodomain of gp41, the transmembrane fusion protein of HIV, without the fusion peptide region was expressed using pET system in E. coli. The expressed protein gp41core, was isolated as inclusion body and was purified by ion-exchange chromatography after solubilized in 6M urea. The purified denatured protein was renaturated and the folded domain of gp41core was identified by the presence of the proteolysis resistence domain and a high content of
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