Conformational Study of Hydroxy Protons in $G_{A1}$ by NMR Spectroscopy

Investigation of the structure of the gangliosides has proven to be very important in the understanding of their biological roles such as regulation of differentiation and growth of cells. Unexchanged hydroxyl protons and amide protons in ganglioside are protrude farther from the carbon backbone than the C-linked protons and provides nOe contacts with other protons which can provide additional distance constraints in structural determination. (omitted)