Proceedings of the Korean Society of Applied Pharmacology (한국응용약물학회:학술대회논문집)

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Agouti Gene의 Human Homologue의 Molecular Structure와 Chromosomal Mapping

  • Heajoon Y. Kwon (Biology Division, Oak Ridge National Laboratory) ;
  • Scott J. Bultman (Biology Division, Oak Ridge National Laboratory, The University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences) ;
  • Christiane Loffler (Institut fur Humangenetik Universitat Gottingen) ;
  • Chen, Wen-Ji (Biochemistry Department, Glaxo Inc. Research Institute, Research Triangle Park) ;
  • Paul J. Furdon (Biochemistry Department, Glaxo Inc. Research Institute, Research Triangle Park) ;
  • John G. Powell (Department of Pediatric Endocrinology, Eastern Carolina University) ;
  • Usala, Anton-Lewis (Department of Pediatric Endocrinology, Eastern Carolina University) ;
  • William Wilkison (Biochemistry Department, Glaxo Inc. Research Institute, Research Triangle Park) ;
  • Ingo Hansman (Institut fur Humangenetik Universitat Gottingen)
  • Published : 1996.11.01
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Abstract

mouse chromesome2에 있는 agouti locus는 정상적으로는 털색깔을 조절하는 gene이다. mouse agouti gene은 최근에 cloning 되었고 131 amino acid peptide와 consensus signal peptide를 encode한다고 보고되었다. 이 논문에서 interspecies-DNA hybridization approach를 이용하여 mouse agouti gene의 human homologue를 cloning 하였다. Sequence analysis 결과, 이는 mouse gene에 85% 유사하였고 consensus signal peptide sequence 를 포함하는 132 amino acid를 coding하였다. somatic-cell hybrid mapping pannel과 Fluorescence-in-situ hybridization에 의한 chromosomal mapping을 한 결과, agouti gene은 MODY (maturity onset diabetes of the young), myeloid leukemia locus 등이 위치한 human chromosome 20q 11.2에 mapping 되었다. 성인 tissue로부터 추출한 RNA를 이용한 발현연구에 의하면 human agouti gene은 adipose tissue와 teatis에 발현되었다.

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