Proceedings of the KOSOMBE Conference (대한의용생체공학회:학술대회논문집)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Electrical characteristics of voltage-dependent $Ca^{++}$ channel in rat chromaffin cell.

흰쥐 부신수질 세포에서 voltage-dependent $Ca^{++}$ 채널의 전기적 특성에 관한 연구

  • Goo, Yong-Sook (Departments of Physiology, College of Medicine, Chungbuk National University) ;
  • Lee, Tae-Soo (Departments of Biomedical Engineering, College of Medicine, Chungbuk National University) ;
  • Cha, Eun-Jong (Departments of Biomedical Engineering, College of Medicine, Chungbuk National University)
  • 구용숙 (충북대학교 의과대학 생리학교실) ;
  • 이태수 (충북대학교 의과대학 의공학교실) ;
  • 차은종 (충북대학교 의과대학 의공학교실)
  • Published : 1994.05.13
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Abstract

Calcium(Ca) ion plays an important role to trigger the secretion of important neurotransmitters. Since Ca ion flows into the cell thru the ion selective channel, the conductance of which depends on the transmembrane potential, the voltage-dependent characteristic of Ca ion channel is crucial to elucidate the stimulus-secretion coupling of exocytosis. The present study measured the Ca ion currents thru a whole-cell configuration patch at the transmembrane potential clamped at various desired levels in the rat chromaffin cell. The resultant current-voltage relationship was differentiated to obtain dynamic conductance at each clamped voltage. Based on these measured data, five numerical parameters were extracted to reveal electrical properties of Ca ion inflow process thru the voltage-gated channel. The present study can be applied to comparing the electrical characteristics of Ca channel under different experimental conditions. Also, further study is warranted to model the conformational changes of the channel molecules.

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