Proceedings of the Korean Society of Applied Pharmacology (한국응용약물학회:학술대회논문집)

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Development of in vitro Short-term Carcinogenicity Test Method and its Mechanism of Action

  • Cho, Dae-Hyun (Department of Toxicology National Institute of Safety Research) ;
  • Kim, Jun-Gyou- (Department of Toxicology National Institute of Safety Research) ;
  • Ahn, Mi-Young (Department of Toxicology National Institute of Safety Research) ;
  • Park, Mi-Kyung- (Department of Toxicology National Institute of Safety Research) ;
  • Moon, Byung-Woo (Department of Toxicology National Institute of Safety Research) ;
  • Moon, Hwa-Hwey (Department of Toxicology National Institute of Safety Research) ;
  • Lee, Byung-Mu- (College of Pharmacy, Sung Kyun Kwan University)
  • Published : 1994.04.01
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Abstract

In order to develope the in vitro short term screen-ing method for carcinogen, we studied a purification method for thymine glycol in oxidaized DNA. Thymine glycol (5,6-dihydroxy-5, 6-dihydrothymine) is the major stable radiolysis poduct in thymine by chemical oxidants and ionzing radiation and it is a useful biomarker among oxidized DNA adducts, related with carcinogenests. Standard thymine glycol was prepared by oxidation of 〔$^3$H〕 thymine with KMnO$_4$ followed by purification with HPLC-LSC system and it was assayed by TLC and gas chromatography-MSD. 〔$^3$H〕 DMA adducts was isolated from E. coli (wild type ) treated with oxidative agents such as benzo(a)pyrene, adriamycin, aflatoxin B$_1$ and KBrO$_3$. These oxidative agents generated free radicals in cells by oxidative metabolism. As a result, thymine glycol was produced in cultured E. coli by four chemicals. This result shows that this methodology should be useful tool in screening oxidative carcinogen.

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