Proceedings of the Korean Society of Applied Pharmacology (한국응용약물학회:학술대회논문집)
- 1994.04a
- /
- Pages.153-163
- /
- 1994
IN VITRO DRUG METABOLISM BY A HUMAN P450 EXPRESSION SYSTEM USING VACCINIA VIRUS.
- S.Ono (Research and Development Laboratory, Amersham K. K.) ;
- Ha, T.tanaka (Research and Development Laboratory, Amersham K. K.) ;
- H.Hotta (Research and Development Laboratory, Amersham K. K.) ;
- M.Tsutsui (Research and Development Laboratory, Amersham K. K.) ;
- F.J.Gonzalez (Laboratory of Molecular Carcinogenesis, National Cancer Institute, National Institutes of Health) ;
- T.Aoyama (Department of Biochemistry, School of Medicine, Shinshu University) ;
- T.Satoh (Laboratory of Biochemical Pharmacology and Biotoxicology, Faculty of Pharmaceutical Sciences, Chiba University)
- Published : 1994.04.01
Abstract
P450s are a superfamily of heme-containing monooxygenases and important in the metabolism of numerous physiological substrates and foreign compounds. It has been established that tilers are at least 30 distinct human isoforms of P450. Four families containing numerous individual P450s are mainly responsible for metabolizing foreign compounds, A cDNA expression system in which individual human P450s are synthesized in cultured human hepatoma (Hep G2) cells infected with a recombinant vaccinia virus containing human P450 cDNA has been constructed.
Keywords