한국응용약물학회:학술대회논문집 (Proceedings of the Korean Society of Applied Pharmacology)

한국응용약물학회 (The Korean Society of Applied Pharmacology)
권호 표지

APPLICATION OF THREE DIMENSIONAL CULTURE OF ADULT RAT HAPATOCYTES IN POLYURETHANE FOAM PORES FOR AN ARTIFICIAL LIVER SUPPORT SYSTEM

  • Funatsu, K. (Department of Chemical Engineering, Kyushu University) ;
  • Matsushita, T. (Department of Chemical Engineering, Kyushu University) ;
  • Ijima, H. (Department of Chemical Engineering, Kyushu University) ;
  • Iwahashi, T. (Department of Chemical Engineering, Kyushu University)
  • 발행 : 1994.04.01
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초록

Spherical multicellular aggregates of adult rat hepatocytes (spheroid) which have tissue like structure, were formed and immobilized in the pores of polyurethane foam (PUF) which was used as a culture substratum. These hepatocyte/spheroids, about 100 $\mu\textrm{m}$ in diameter, have maintained higher differentiated functions than those of hepatocyte/monolayer for about 3 weeks in serum-free medium. Then, we designed a prototype module of an artificial liver support system using a PUF/spheroid packed-bed, in which hepatocyte/spheroids were immobilized at high density. The urea synthesis activity of the artificial liver was maintained at least 10 days in 100% rat blood plasma. We start examining the performance of hybrid artificial liver in an ex vivo extracorporeal experiment with an acute hepatic failure rat.

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