Proceedings of the Korean Society of Applied Pharmacology (한국응용약물학회:학술대회논문집)

The Korean Society of Applied Pharmacology (한국응용약물학회)
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APPLICATION OF THREE DIMENSIONAL CULTURE OF ADULT RAT HAPATOCYTES IN POLYURETHANE FOAM PORES FOR AN ARTIFICIAL LIVER SUPPORT SYSTEM

  • Funatsu, K. (Department of Chemical Engineering, Kyushu University) ;
  • Matsushita, T. (Department of Chemical Engineering, Kyushu University) ;
  • Ijima, H. (Department of Chemical Engineering, Kyushu University) ;
  • Iwahashi, T. (Department of Chemical Engineering, Kyushu University)
  • Published : 1994.04.01
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Abstract

Spherical multicellular aggregates of adult rat hepatocytes (spheroid) which have tissue like structure, were formed and immobilized in the pores of polyurethane foam (PUF) which was used as a culture substratum. These hepatocyte/spheroids, about 100 $\mu\textrm{m}$ in diameter, have maintained higher differentiated functions than those of hepatocyte/monolayer for about 3 weeks in serum-free medium. Then, we designed a prototype module of an artificial liver support system using a PUF/spheroid packed-bed, in which hepatocyte/spheroids were immobilized at high density. The urea synthesis activity of the artificial liver was maintained at least 10 days in 100% rat blood plasma. We start examining the performance of hybrid artificial liver in an ex vivo extracorporeal experiment with an acute hepatic failure rat.

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