고려인삼학회:학술대회논문집 (Proceedings of the Ginseng society Conference)

고려인삼학회 (The Korean Society of Ginseng)
권호 표지

DIFFERENTIATION MECHANISM OF GINSENOSIDES IN CULTURED MURINE F9 TERATOCARCINOMA STEM CELLS

  • Lee H.Y. (Department of Molecular Biology, Pusan National University, the Cancer Research Center) ;
  • Kim S.I. (Korea Ginseng and Tobacco Research Institute) ;
  • Lee S.K. (College of Pharmacy and the Research Center for New Drug Development, Seoul National University) ;
  • Chung H.Y. (Department of Phamacy, Pusan National University) ;
  • Kim K.W. (Department of Molecular Biology, Pusan National University, the Cancer Research Center)
  • 발행 : 1993.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The effects of total ginseng saponin. extracts of Panax ginseng C.A. Meyer, on the differentiation of F9 teratocarcinoma stem cells were studied. F9 stem cells cultured in the presence of ginseng saponin together with dibutyric cAMP became parietal endoderm - like cells. Moreover, the expressions of differentiation marker genes. laminin. type IV collagen. and retinoic acid $receptor-{\beta}(RAR{\beta})$ were increased after treatment of ginseng saponin. Among various ginsenosides purified from crude ginseng saponin, $Rh_1\;and\;Rh_2$ caused the differentiation of F9 cells most effectively. Since ginsenosides and steroid hormone show resemblance in chemical structure. we studied the possibility of the involvement of a steroid receptor in the differentiation process induced by ginsenosides. According to Southwestern blot analysis, a 94 kDa protein regarding as a steroid receptor was detected in F9 cells cultured in the medium containing ginseng saponin. Based on these data, we suggest that ginseng saponin, especially ginsenosides $Rh_1\;and\;Rh_2$ cause the differentiation of F9 cells and the effects of ginsenosides might be exerted via binding with a steroid receptor or its analogous nuclear receptor.

키워드