Physiological and Nutritional Factors for Efficient Sporulation and Toxin Formation in Bacillus tthuringiensis

In order to optimally induce sporulation and toxin formation in Bacillus thuringiensis, exhaustion of specific nutrients as well as resuspension experiments were tried. Sporulation and toxin formation was most abunduntly occurred when the growth was limited by carbon source. It was also occurred in a resuspension medium containing only distilled water. Various environmental and physiological factors affecting the efficiencies of spore and toxin formation were examined in chemically defined media. As a result of these studies, a batch fermentation resulted in higher spore and toxin yield than ever reported