Proceedings of the Korean Society for Applied Microbiology Conference (한국미생물생명공학회:학술대회논문집)
- 1986.12a
- /
- Pages.515.2-515
- /
- 1986
Cloning and Expression of B. Aphaericus insecticidal toxin gene in E. coli
- Lim, Pyong-Ok (The Inst. for Genetic Engineering, Dept. of Biology, Kohkuk Univ.) ;
- Lee, Hong-Sup (The Inst. for Genetic Engineering, Dept. of Biology, Kohkuk Univ.) ;
- Lee, Hyung-Hoan (The Inst. for Genetic Engineering, Dept. of Biology, Kohkuk Univ.)
- Published : 1986.12.01
Abstract
B. sphaericus 1593 K-5 synthesize a potent entomicidal toxin against mosquito larvae. B. sphaericus EcoRl DNA fragment carrying the biocidal activity was clonied and expression in E. coli JM83. For the construction of a recombinant plasmid bearing the toxin activity the DNA of B. sphaericus was partially digested by the EcoRl. The EcoRl DNA fragments were ligated to plasmid pUC 8-EcoR1 site. The transformants were selected on LB plates containing X-gall and ampicilline. The transformants were bioassayed against mosquito larvae of which two clones showed biocidal activity. The two clones were redigested with Eco R1 and analyzed by 0.7% agarose gel electrophorsis.
Keywords