Proceedings of the Korean Society for Applied Microbiology Conference (한국미생물생명공학회:학술대회논문집)
- 1986.12a
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- Pages.514.2-514
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- 1986
Factors affecting the final antibiotic titer of sisomicin fermentation
Abstract
Since sisomicin which is produced by Micromonospora inyoensis is an intracellular antibiotic, the final antibiotic titer to be attained depends singnificantly on the cell mass in fermentation broth. Cobalt ion in medium was indispensable for getting a high antibiotic titer. However, in the presence of cobalt ion in medium, the antibiotic production proceeded up to about 4 days and thereafter stopped. From the experiments on theaddition of cobalt ion to culture medium, it was shown that the antibiotic production stopped due to the other physiological properties of cells rather than the accumulation of antibiotic in cells.
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