Abstract
The effect of the saponin fraction extracted from Panax ginseng C.A. Meyer on the antioxidant activity of ${\alpha}-tocopherol$ was investigated in vitro as well as in vivo. Microsomal preparation of albino rat (Sprague-Dawley, 180-200g) was incubated in the mixture containing NADPH, $Fe^{3+},$ ATP, ${\alpha}-tocopherol$ with and/or without ginseng saponin fraction for 30 minutes and the malondialdehyde formed was assayed and found that the saponin fraction stimulated the antioxidant activity of ${\alpha}-tocopherol$ cooperatively. It was also realized that the cooperative stimulation of the antioxidant activity of ${\alpha}-tocopherol$ was most eminent when the concentration of the saponin fraction was around $10^{-5}\%$ in the reaction mixture. Alcoholic suspension of ${\alpha}-tocopherol$ with and/or without ginseng saponin fraction was administered orally to rats in which the lipid peroxidation was induced by ethanol administration and the lipid peroxide contents of the liver were assayed at certain periods of time after ${\alpha}-tocopherol$ administration in this animal. It was reported that the saponin fraction stimulated the absorption of ${\alpha}-tocopherol$ in rats and this was confirmed again in the present work. From the previous work and present experimental results, it seemed that the saponin fraction accelerated the absorption of ${\alpha}-tocopherol$ and therefore stimulated the antioxidant activity of ${\alpha}-tocopherol$ more effectively in the animal body.
한국산 인삼(Panax ginseng C. A. Meyer) 뿌리에서 추출한 사포닌 성분이 ${\alpha}$-tocopherol의 항산화 활성에 미치는 영향을 실험관 내 실험과 동물실험으로 관찰하였다. 흰쥐(Sprague-Dawley, male, $18{\~}200g$)의 마이크로좀 분획을 NADPH, $Fe^{+3}$, ATP $\alpha^{-+}$-ocopherol 과 인삼 사포닌 분획 (대조군은 사포닌 분획대신 같은 부피의 물)을 함유한 혼합물에서 30분간 반응을 진행시키고 생성되는 molondialdehyde를 분석한 결과 사포닌 분획은 ${\alpha}$-tocopherol의 항산화작용을 상승적으로 증가시켰다. 그리고 ${\alpha}$-tocopherol의 항 산화작용에 상승적 효과는 반응액에서의 인삼 사포닌 분획의 농도가 $10^{-5}\%$ 전후에서 특히 현저하였다. 술로 과산화물을 유발케 한 쥐에게 ${\alpha}$-tocopherol만을 또는 ${\alpha}$-tocopherol과 인삼 사포닌 분획의 혼합물을 경구투여하고 일정 시간 후에 간의 과산화 지질을 thiobarbituric acid 방법으로 분석한 결과 사포닌 분획이 동물체내에서도 ${\alpha}$-tocopherol의 항 산화작용을 크게 촉진시키는 것으로 관찰되었다. 또한 사포닌 분획은 ${\alpha}$-tocopherol의 흡수를 촉진시키는 것으로 보고되고 있으며 본 실험에서도 이것을 확인하였고 이와 같은 사포닌 분획의 ${\alpha}$-tocopherol흡수 촉진은 ${\alpha}$-tocopherol의 항 산화작용을 더욱 효과적으로 촉진 시킬 것으로 생각된다.