Proceedings of the Korean Society for Applied Microbiology Conference (한국미생물생명공학회:학술대회논문집)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification of Low Mole-cular Weight Endo-glucanase from Cellulase and Its Action on Cellulose

  • Ryu, Wang-Shick (Dept. of Biol. Sci. & Eng. K.A.I.S.) ;
  • Ryu, D.Y. (Dept. of Biol. Sci. & Eng. K.A.I.S.)
  • Published : 1979.10.01
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Abstract

Low molecular weight endo-glucanase fraction of cellulase from Trichoderma reesei was purified using Sephadex G-100 and concanavalin A-Sepha-rose 4B affinity chromatography. Its biochemical characteristics including pH profile, temperature profile and kinetic behavior were studied. The optimum conditions for enzymatic reaction were pH 6.0 and $5^{\circ}C.$ The activation energy for CMC (carboxymethylcellulose) was 10,800 cal/mole. Its adsorption to amorhous and crystalline cellulose was observed. Adsorption to amorphous cellulose was more rapid and greater than that of crystalline cellulose. Reconstitution study was performed. Significance of low molecular weight endo-glucanase on cellulose hydrolysis will be further discussed.

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