Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Characterization of Pyribenzoxim Metabolizing Enzymes in Rat Liver Microsomes

  • Liu Kwang-Hyeon (Department of Pharmacology and Pharmaco Genomics Research Center, Inje University College of Medicine) ;
  • Moon Joon-Kwan (School of Agricultural Biotechnology, Seoul National University) ;
  • Seo Jong-Su (Department of Molecular Biosciences and Bioengineering, University of Hawaii) ;
  • Park Byeoung-Soo (School of Agricultural Biotechnology, Seoul National University) ;
  • Koo Suk-Jin (LG Life Sciences Ltd.) ;
  • Lee Hye-Suk (College of Pharmacy, Wonkwang University) ;
  • Kim Jeong-Han (School of Agricultural Biotechnology, Seoul National University)
  • Published : 2006.03.01
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Abstract

The primary metabolism of pyribenzoxim was studied in rat liver microsomes in order to identify the cytochrome P450 (CYP) isoform(s) and esterases involved in the metabolism of pyribenzoxim. Chemical inhibition using CYP isoform-selective inhibitors such as ${\alpha}$-naphthoflavone, tolbutamide, quinine, chlorzoxazone, troleandomycin, and undecynoic acid indicated that CYP1A and CYP2D are responsible for the oxidative metabolism of pyribenzoxim. And inhibitory studies using eserine, bis-nitrophenol phosphate, dibucaine, and mercuric chloride indicated pyribenzoxim hydrolysis involved in microsomal carboxylesterases containing an SH group (cysteine) at the active center.

Keywords

References

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